Electroporation uses electrical pulses to create temporary pores in cell membranes, allowing substances like nucleic acids, proteins, or drugs to enter cells. This process is also known as electropermeabilization.
In cell therapy, electroporation is used to transport genetic material (DNA and RNA) or other molecules into cells without using a virus. Once inside, these molecules can act on the cell, and the genetic material may either remain independent or become integrated into the host genome depending on the downstream experimental steps.
Electroporation can be used in different ways (in vitro, in vivo, ex vivo) and has been successfully used in various applications. For instance, it has been used to produce CAR-T cells via mRNA targeting of melanomas at a clinical scale.
Furthermore, electroporation can be used to avoid the toxicity and immunogenicity risks associated with adenoviral and lentiviral vectors in gene therapy for both in vitro and ex vivo settings. Cells created using electroporation are better tolerated and can be used repeatedly with less risk to patients.
Electroporation is a vital tool in cell therapy due to its versatility, safety, and cost-effectiveness. EHT pulse generators can help companies scale-up cell therapies to reduce the cost and increase access of these treatments.